Re: Alk Phos osteoblast staining
Ryan,
the problem with frozens for alk. p is that there is usually so much of it
that it is all over the place. yes it is as simple as incubating in nbt/bcip
with the proper ph, i just suggest for frozens you do it quickly and more
dilute.
Patsy
Ryan Blair Rountree wrote:
> Can anyone send me a protocol for staining osteoblasts with Alkaline
> Phosphatase? I am planning to do this on fixed frozen tissue that is from
> young enough mice that I can still section tissue just beginning to
> ossify. Is it as simple as just sectioning the tissue and incubating it
> in NBT/BCIP? Or are there other complexities?
>
> Thanks for any input!!
>
> Ryan
>
> Ryan Rountree
> Department of Developmental Biology
> B301, Beckman center
> 279 Campus Drive
> Stanford, CA 94305
> (650) 725-7599
> Fax: (650) 725-7739
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