Re: Alk Phos osteoblast staining

From:Patsy Ruegg

the problem with frozens for alk. p is that there is usually so much of it
that it is all over the place.  yes it is as simple as incubating in nbt/bcip
with the proper ph, i just suggest for frozens you do it quickly and more

Ryan Blair Rountree wrote:

> Can anyone send me a protocol for staining osteoblasts with Alkaline
> Phosphatase?  I am planning to do this on fixed frozen tissue that is from
> young enough mice that I can still section tissue just beginning to
> ossify.  Is it as simple as just sectioning the tissue and incubating it
> in NBT/BCIP?  Or are there other complexities?
> Thanks for any input!!
> Ryan
> Ryan Rountree
> Department of Developmental Biology
> B301, Beckman center
> 279 Campus Drive
> Stanford, CA 94305
> (650) 725-7599
> Fax: (650) 725-7739

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