Re: Alcian Blue staining problem
Try to rinse your slides with buffer (at the same pH than your
staining solution). If possible, you also should mount your slides
with a buffered mounting medium or a quick drying medium (so that it
will not modify your stain)...
These are the experience I get when I was studying European beech
bark (phloem, cambium) and wood sample mounted in JB-4 resin...
Hope it helps
Division of viral pathogenesis
Beth Israel deaconess Medical Center
330 Brookline Avenue
Boston, MA 02215
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