Hi Manisha,

Try to rinse your slides with buffer (at the same pH than your 
staining solution). If possible, you also should mount your slides 
with a buffered mounting medium or a quick drying medium (so that it 
will not modify your stain)...

These are the experience I get when I was studying European beech 
bark (phloem, cambium) and wood sample mounted in JB-4 resin...

Hope it helps
-- 
Christian Wuethrich
Division of viral pathogenesis
Beth Israel deaconess Medical Center
330 Brookline Avenue
Boston, MA 02215
United states