RE: mouse tissue processing

From:Cynthia Favara


I think 15 hours is definitely too long. Specifics depend on type of
processor and size of tissue. I have used a Shandon Citadel for years. If
you would like specifics let me know but I would do no longer than 30min
each station, four paraffin!

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Martin, Ronald []
Sent: Tuesday, October 08, 2002 7:30 AM
To: ''
Subject: mouse tissue processing

Fellow techs,
How long of a processing schedule would you use for mouse skin, pancreas and
salivary glands? 8 hours? 9 hours? 15 hours? A post doc I am doing work for
requested  a 15 hour processing schedule  now the tissue (esp. pancreas) is
dry (not overly dry but dry enough) and is difficult to section. I think a
shorter processing schedule will make the tissue easier to section. Any

Thanks in advance,
Ron Martin

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