From: | "Maximova, Olga *" |
Hi,
I am just new here...
Concerning
"decontamination" of prion protein in CJD brain:
Ned Tijdschr
Geneeskd 1999 Jul 17;143(29):1511-4
[Safety measures for handling
laboratory specimen and patients with Creutzfeldt-Jakob disease]
[Article
in Dutch]
Van Everbroeck B, Pals P, Cras P.
Universitaire
Instelling Antwerpen, Born Bunge Stichting, laboratorium Neurobiologie, Wilrijk,
Belgie.
Creutzfeldt-Jakob disease (CJD) is a transmissible subacute
spongiform encephalopathy that invariably leads to death. The presumed causative
agent, the prion protein, is highly resistant to inactivation and has a long
incubation period. Physical contact with CJD patients (as in clinical care)
entails no risk of transmission. During procedures such as lumbar puncture where
contact with infected material is possible, precautions are necessary.
Precautions are: the use of gloves, maximal protection of people who come in
contact with contaminated tissue (e.g. pathologist and histological laboratory
worker) and transportation of samples in a closed and labelled container. For laboratory research the tissue must be
submerged in 92-98% formic acid for 1 hour. All used materials
and instruments must be decontaminated properly, using for instance NaOH, NaClO,
guanidine thiocyanate or steam autoclaving. If adequate precautions are taken
contact with contaminated materials can be safe.
Olga Maximova, M.D.,
Ph.D.
CBER/FDA
HFM-470
1401 Rockville Pike
Rockville, MD
20852-1448
Tel: (301) 827-2852
-----Original
Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent:
Wednesday, October 16, 2002 4:09 PM
To: Anacko, Nancy L.
Cc: 'Server,
Histonet'
Subject: Re: cjd brain
it is strong formic acid, 40% i
think.
patsy
"Anacko, Nancy L." wrote:
> hi...the
neuropathologist here wants to put a WHOLE cjd brain in formic acid
> and
he told me that there is a certain dilution the formic acid has to be
>
...........does anyone out there in histonet land know this dilution
?????
> thanks,
> nancy