RE: TSE Labs
doing CWD --- this is our first season --- What I have done is the
1) Wear disposable lab coats (Safety Plus
Products, 4123 Terminal Drive,Suite 210, MacFarland WI, 53558 sorry I don't have
2) Wear nitrile gloves at all
3) Microtome table is covered with
disposable lab bench paper and changed daily
4) Use disposable blades and put in a
5) EVERYTHING --- paraffin shavings, lab
coats, gloves, papers, EVERYTHING -- is put in a biohazard bag. I
purchased a tall kitchen waste can at a local store and use that for my
biohazard can. I use biohazard bags ordered from Fisher scientific to line
it with. This is emptied after each day of use. I keep a red
biohazard can with a step-on lever to open the lid. I line this
can with a red biohazard bag to put the waste biohazard bag in so I
can take it all to the incinerator. Inside the red can is considered
clean, so there is nothing on the outside that can contaminate the area.
6) I use a disposable covered plastic food storage dish
(Rubbermaid oblong dish) for my waterbath. I put this in the regular
waterbath, but the sections are floated only in this disposable dish. When
I'm finished cutting, I put the lid on this dish (which seals tightly) and put
it in the waste biohazard bag, which prevents the water from leaking out of the
biohazard bag on the way to the incinerator.
7) I keep all deparafinzing, peroxidase blocking and
concentrated formic acid pretreatments in a hood in the lab on a rubber
mat. I use paper toweling to surround the reagents and these are disposed
of at day's end.
this helps! good luck
Verinary Diagnostics Lab
I'm hoping someone out there can
help me. We are in the process of setting up a histology lab that will
be dedicated solely to checking for CWD in brain tissue, and I'd love to
brainstorm with others who work in labs that deal with the prion
diseases. Specifically I'm interested in some of the safety equipment,
suppliers, as well as any good tips for easier sectioning of brain tissue
post-fixation in formic acid.
If anyone out there is currently working
with this tissue, and wouldn't mind answering a few questions, please let me
Thanks in advance!!
University of MN
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