RE: Paragon stain source
I notice that the color index for the Basic Fuchsin in this formula is
42510. Would that be the same color index for Basic Fuchsin in Kinyoun's AFB
stain?
Respectfully,
Bruce Gapinski HT(ASCP)
-----Original Message-----
From: FreidaC@aol.com [mailto:FreidaC@aol.com]
Sent: Wednesday, October 09, 2002 4:41 PM
To: mcauliff@UMDNJ.EDU; Cathy@wasatchhisto.com
Cc: histonet@pathology.swmed.edu
Subject: Re: Paragon stain source
I worked with Martin et al. and the sodium borate was added
to the Paragon
stain to help the penetration into epon embedded sections.
The Paragon stain
was designed for frozen sections and was marketed by a
company by the name of
Paragon. The formula that appeared on the bottles of
Paragon stain was as
follows:
Toluidine blue CI 520040 ................0.73 gm
Basic fuchsin , CI 42510 ................0.27 g,
Ethyl alcohol, 30% ........................100 ml
If adding sodium borate, it must not be added to the bottle,
as it affects
the stability, but is added to the stain on the slide - only
a little pinch.
Hope this helps.
Freida Carson
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