RE: Paragon stain source

From:Bruce Gapinski

I notice that the color index for the Basic Fuchsin in this formula is
42510. Would that be the same color index for Basic Fuchsin in Kinyoun's AFB
stain? 
Respectfully,
	Bruce Gapinski HT(ASCP)

		-----Original Message-----
		From:	FreidaC@aol.com [mailto:FreidaC@aol.com]
		Sent:	Wednesday, October 09, 2002 4:41 PM
		To:	mcauliff@UMDNJ.EDU; Cathy@wasatchhisto.com
		Cc:	histonet@pathology.swmed.edu
		Subject:	Re: Paragon stain source

		I worked with Martin et al. and the sodium borate was added
to the Paragon 
		stain to help the penetration into epon embedded sections.
The Paragon stain 
		was designed for frozen sections and was marketed by a
company by the name of 
		Paragon.  The formula that appeared on the bottles of
Paragon stain was as 
		follows:
		Toluidine blue  CI 520040 ................0.73 gm
		Basic fuchsin , CI 42510 ................0.27 g,
		Ethyl alcohol, 30% ........................100 ml

		If adding sodium borate, it must not be added to the bottle,
as it affects 
		the stability, but is added to the stain on the slide - only
a little pinch.

		Hope this helps.

		Freida Carson



<< Previous Message | Next Message >>