RE: Daily Digest
When I asked about histology at home I did not mean a full histology lab
with all types of flamable liquids and carcinogens (laughing). What I have
heard is that people simply cut blocks at home which would just consist of
a microtome and waterbath and an oven to bake the slides. I guess I should
clarify before more panic sets in.
--- Felisha Sandifer
--- leish27@earthlink.net
--- EarthLink: The #1 provider of the Real Internet.
> [Original Message]
> From: HistoNet Server
> To: HistoNet Server
> Date: 10/8/2002 12:59:38 AM
> Subject: Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 02:31:01 -0500
> From: louise renton
> Subject: Re: Soap
>
> Would heat and solvents of normal processing not alter the structure?
What
> about frozen sections?
>
> >From: marjorie lehman
> >To: histonet
> >Subject: Soap
> >Date: Fri, 04 Oct 2002 12:06:06 -0400
> >
> >Hi,
> >Does anyone have any suggestions/methods/thoughts on sectioning a piece
of
> >soap? Actually, it is not a true soap, it's a detergent bar/"Beauty Bar"
> >(whatever) and the investigator is looking for structure after the bar
has
> >been
> >extruded (formed into a cake). Oh my, I do get asked to cut strange
> >things!!
> >Thanks,
> >Marge Lehman
>
>
>
>
> Louise Renton
> Bone Research Unit
> MRC
> Johannesburg
> South Africa
> Tel & fax +27 11 717 2298
> "Time flies like an arrow, fruit flies like a banana"
>
>
> _________________________________________________________________
> Join the worldis largest e-mail service with MSN Hotmail.
> http://www.hotmail.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 07:15:47 -0500
> From: "Morken, Tim"
> Subject: New or imporved RE: National COnference
>
>
> Cheryl wrote: < for those of us who couldn't make it?>>
>
> I learned that Rockbottoms in Long Beach has a very good Octoberfest
lager.
> And the smoked-chicken enchiladas were good as well.
>
>
> Tim
> Atlanta
>
> - -----Original Message-----
> From: Cheryl Powell [mailto:cherylhisto@hotmail.com]
> Sent: Sunday, October 06, 2002 5:06 PM
> To: histonet@pathology.swmed.edu
> Subject: National COnference
>
>
> Hi everybody! So how was the NSH national meetings? COUld people share
> something they learned new or improved for those of us who couldn't make
it?
>
>
>
> Cheryl Ann Powell B.S., HTL(ASCP)
> Botsford General Hospital
> Farmington Hills, MI USA
>
> _____
>
> Chat with friends online, try MSN Messenger: Click
> Here
>
>
>
>
>
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>
> class=855494911-07102002> face="Times New Roman" color=#000000 size=3>Cheryl wrote: <<Could
people
>
> share something they learned new or improved for those of us who couldn't
make
>
> it?>>
> I
> learned that Rockbottoms in Long Beach has a very good Octoberfest lager.
And
> the smoked-chicken enchiladas were good as well.
> class=855494911-07102002>
> class=855494911-07102002>
> class=855494911-07102002>Tim
> class=855494911-07102002>Atlanta
>
>
>
>
>
Hi everybody! So how was the NSH national
meetings?
> COUld people share something they learned new or improved for those of
us
> who
> couldn't make it?
>
Cheryl Ann Powell B.S.,
> HTL(ASCP)
Botsford
> General Hospital
>
>
Farmington Hills,
MI
> USA
>
>
> Chat with friends online, try MSN Messenger: href="http://g.msn.com/1HM1ENUS/c144??PS=47575">Click
> Here
>
> - --Boundary_(ID_BxzfiSuYpVGrhuPAZlK1gg)--
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 07:30:15 -0500
> From: rkline@emscience.com
> Subject: Re: Buffer recipe
>
>
> Marina,
> I'm not sure Soerensen's buffer is the proper buffer to Giemsa. The salt
> combinations in buffers can effect staining. Have you tried the Gurr
> tablets from Merck?
> Rande Kline
> EM Science
>
>
>
>
> marina goumenou on 10/05/2002 04:25:47 PM
>
> To: histonet@pathology.swmed.edu
> cc:
> Subject: Buffer recipe
>
>
>
> Hello to everybody!
>
> Your suggestions for my May Grunwald - Giemsa problems were really very
> helpfull. However, I stained my slides with dye solutions in Soerensen
> buffer pH 6,8 but they appeared to be too blue. So I intent to use even
> lower pH value (something like 6,0 or 6,2). Does anybody have a recipe for
> apropriate buffer (Soerensen or anything you think) in these pH values?
> Thanking you in advance
> Best Regards
> Marina Goumenou
> Athens - Greece
>
>
> _________________________________________________________________
> Join the worldis largest e-mail service with MSN Hotmail.
> http://www.hotmail.com
>
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 08:30:09 -0500
> From: "Johnston, Kathy"
> Subject: RE: Buffer recipe
>
> This is my table:
>
> You have your 2 stock buffer solutions:
> 1. M/15 sodium phosphate dibasic (Na2HPO4) - dissolve 9.465 g in 1L
dH2O.
> 2. M/15 potassium acid phosphate (KH2PO4) - dissolve 9.08 g in 1L dH2O.
>
> To make the amounts of each solution follow the below table:
>
> pH M/15 KH2PO4 M/15 Na2HPO4
> mL mL
> 8.0 4 96
> 7.6 12 88
> 7.2 27 73
> 6.8 50 50
> 6.4 71 29
> 6.0 88 12
> 5.6 95 5
>
> Hope this helps!
>
> Kathy Johnston
> Tech II - Special Stains
> Anatomic Pathology - Foothills Medical Center
> Calgary Laboratory Services
> Ph - 403-944-4760
> Fax - 403-270-4093
> kathy.johnston@cls.ab.ca
>
> - -----Original Message-----
> From: marina goumenou [mailto:mtg67@hotmail.com]
> Sent: Saturday, October 05, 2002 2:26 PM
> To: histonet@pathology.swmed.edu
> Subject: Buffer recipe
>
>
>
> Hello to everybody!
>
> Your suggestions for my May Grunwald - Giemsa problems were really very
> helpfull. However, I stained my slides with dye solutions in Soerensen
> buffer pH 6,8 but they appeared to be too blue. So I intent to use even
> lower pH value (something like 6,0 or 6,2). Does anybody have a recipe
for
> apropriate buffer (Soerensen or anything you think) in these pH values?
> Thanking you in advance
> Best Regards
> Marina Goumenou
> Athens - Greece
>
>
> _________________________________________________________________
> Join the worldis largest e-mail service with MSN Hotmail.
> http://www.hotmail.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 08:30:29 -0500
> From: "Johnston, Kathy"
> Subject: RE: High Iron Diamine stain for sulphomucins
>
> This is what I have found, although I believe the chemicals to be quite
> toxic to use.
>
> http://www.bris.ac.uk/pathandmicro/cpl/hidab.html
>
> Enjoy!
>
> Kathy Johnston
> Tech II - Special Stains
> Anatomic Pathology - Foothills Medical Center
> Calgary Laboratory Services
> Ph - 403-944-4760
> Fax - 403-270-4093
> kathy.johnston@cls.ab.ca
>
>
> - -----Original Message-----
> From: Martin, Ronald [mailto:Ronald.Martin@umassmed.edu]
> Sent: Friday, October 04, 2002 12:14 PM
> To: histonet@pathology.swmed.edu
> Subject: High Iron Diamine stain for sulphomucins
>
>
> Fellow techs,
> Does anyone have a procedure they are willing to share for a High Iron
> Diamine stain for sulphomucins? This is new to me so I will new
information
> regarding times, concentrations, chemicals etc. We are trying to
> differentiate between sulfated and carboxylated components in mouse
> stomach/intestine.
> Thanks in advance,
> Ron Martin
> Research Associate
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 10:00:26 -0500
> From: "Weems, Joyce"
> Subject: HistoQIP Program
>
> Has anyone seen what is required for this program? I believe it would be
> more better than the Immuno program, but would like to hear from you
before
> I order! Thanks, j
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
> 404-851-7376
> 404-851-7831 - fax
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 10:30:17 -0500
> From: "ander093@tc.umn.edu"
> Subject: Re: HistoQIP Program
>
> Hi all,
> I am interested in this program as well. If anyone has any info.....would
you
> please reply to the list. Thank you.
>
> LuAnn Anderson
>
> >
> > Has anyone seen what is required for this program? I believe it would be
> > more better than the Immuno program, but would like to hear from you
before
> > I order! Thanks, j
> >
> >
> > Joyce Weems
> > Pathology Manager
> > Saint Joseph's Hospital of Atlanta
> > 404-851-7376
> > 404-851-7831 - fax
> >
> >
> >
> > .
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 10:46:03 -0500
> From: "Weems, Joyce"
> Subject: RE: HistoQIP Program
>
> Oops, for anyone checking grammar I was testing you! :>)
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
> 404-851-7376
> 404-851-7831 - fax
>
> -----Original Message-----
> From: Weems, Joyce
> Sent: Monday, October 07, 2002 10:51 AM
> To: 'Histonet'
> Subject: HistoQIP Program
>
> Has anyone seen what is required for this program? I believe it would be
> more better than the Immuno program, but would like to hear from you
before
> I order! Thanks, j
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
> 404-851-7376
> 404-851-7831 - fax
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 11:15:10 -0500
> From: Christian Wuthrich
> Subject: Re: Alcian Blue staining problem
>
> Hi Manisha,
>
> Try to rinse your slides with buffer (at the same pH than your
> staining solution). If possible, you also should mount your slides
> with a buffered mounting medium or a quick drying medium (so that it
> will not modify your stain)...
>
> These are the experience I get when I was studying European beech
> bark (phloem, cambium) and wood sample mounted in JB-4 resin...
>
> Hope it helps
> - --
> Christian Wuethrich
> Division of viral pathogenesis
> Beth Israel deaconess Medical Center
> 330 Brookline Avenue
> Boston, MA 02215
> United states
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 12:00:20 -0500
> From: pruegg@colobio.com
> Subject: Re: New or imporved RE: National COnference
>
> I learned that it is bad luck to watch a Bronco monday night football
> game from the Rock Bottom brewery in Long Beach, seriously though, when
> I get a chance
> I will put together a synopsis of some important things I learned at the
> NSH S/C 2002.
> Patsy Ruegg
>
> "Morken, Tim" wrote:
>
> > Cheryl wrote: < > improved for those of us who couldn't make it?>>
> > I learned that Rockbottoms in Long Beach has a very good Octoberfest
> > lager. And the smoked-chicken enchiladas were good as well. TimAtlanta
> >
> > -----Original Message-----
> > From: Cheryl Powell [mailto:cherylhisto@hotmail.com]
> > Sent: Sunday, October 06, 2002 5:06 PM
> > To: histonet@pathology.swmed.edu
> > Subject: National COnference
> >
> > Hi everybody! So how was the NSH national meetings? COUld
> > people share something they learned new or improved for
> > those of us who couldn't make it?
> >
> > Cheryl Ann Powell B.S., HTL(ASCP)Botsford General Hospital
> > Farmington Hills, MI USA
> >
> >
> > -------------------------------------------------------------
> > Chat with friends online, try MSN Messenger: Click Here
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 12:00:36 -0500
> From: pruegg@colobio.com
> Subject: Re: HistoQIP Program
>
> Joyce,
> what immuno program are you refering to?
> we will be accepting the first 300 participents for HistoQip in the
beginning
> and there is a committee designing the program, i think the first survey
will
> be for H&E and trichrome with some goal for expanding into IHC down the
road.
> Freida Carson may be able to enlighten us further on the program content
so
> that we can decide to participate or not. freidac@aol.com
> Patsy
> "Weems, Joyce" wrote:
>
> > Has anyone seen what is required for this program? I believe it would be
> > more better than the Immuno program, but would like to hear from you
before
> > I order! Thanks, j
> >
> > Joyce Weems
> > Pathology Manager
> > Saint Joseph's Hospital of Atlanta
> > 404-851-7376
> > 404-851-7831 - fax
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 12:00:51 -0500
> From: "Weems, Joyce"
> Subject: RE: HistoQIP Program
>
> CAP has a program that we have participated in for a few years. The
problem
> is that 1. it is geared for pathologists and 2. we often do not have all
the
> antibodies designated for testing.
> Joyce
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
> 404-851-7376
> 404-851-7831 - fax
>
> -----Original Message-----
> From: Patsy Ruegg [mailto:pruegg@colobio.com]
> Sent: Monday, October 07, 2002 12:48 PM
> To: Weems, Joyce; freidac@aol.com
> Cc: 'Histonet'
> Subject: Re: HistoQIP Program
>
> Joyce,
> what immuno program are you refering to?
> we will be accepting the first 300 participents for HistoQip in the
> beginning
> and there is a committee designing the program, i think the first survey
> will
> be for H&E and trichrome with some goal for expanding into IHC down the
> road.
> Freida Carson may be able to enlighten us further on the program content
so
> that we can decide to participate or not. freidac@aol.com
> Patsy
> "Weems, Joyce" wrote:
>
> > Has anyone seen what is required for this program? I believe it would be
> > more better than the Immuno program, but would like to hear from you
> before
> > I order! Thanks, j
> >
> > Joyce Weems
> > Pathology Manager
> > Saint Joseph's Hospital of Atlanta
> > 404-851-7376
> > 404-851-7831 - fax
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 13:30:25 -0500
> From: Melissa Jans
> Subject: HT/HTL tissue list
>
>
>
> Does anyone know when the list of tissues will be mailed out for the
HT/HTL
> exam applications that were due on October 1st. We have some anxious
people
> in the lab who want to get started!
>
> Thanks,
>
> Melissa Jans
>
>
>
>
>
> - ---------------------------------
> Do you Yahoo!?
> Faith Hill - Exclusive Performances, Videos, & more
> faith.yahoo.com
>
>
>
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>
> Does anyone know when the list of tissues will be mailed out for the
HT/HTL
> exam applications that were due on October 1st. We have some
> anxious people in the lab who want to get started!
> Thanks,
> Melissa Jans
>
Do you Yahoo!?
> Faith Hill - Exclusive Performances,
> Videos, & more
> faith.yahoo.com
>
> - --Boundary_(ID_3c0lmgWhWMKiFbtvskvHbw)--
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 14:00:48 -0500
> From: CYNTHIA HAYNES
> Subject: lens of rat/mice
>
>
>
> Dear Histonetters,
>
> I would like to know if there is anyone out in H&E land who has
> experience with cutting frozen sections on rat and/or mice lens. I've
tried
> cutting them fresh and fixed and both have left with shattered lens.
Please
> e-mail me with any information on how to section them correctly.
> Sincerely yours,
> Cynthia Haynes
>
>
> P.S. My e-mail address is cynthiahaynes@hotmail.com
>
> _________________________________________________________________
> Join the worldis largest e-mail service with MSN Hotmail.
> http://www.hotmail.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 14:16:11 -0500
> From: FreidaC@aol.com
> Subject: Re: HistoQIP Program
>
> The new HistoQIP program is an external quality assessment program that
is a
> cooperative effort of the NSH and CAP. It is designed specifically for
the
> histotechnology laboratory. There will be two submissions a year, and
each
> will consists of specific H&E's and two special stains. The first
submission
> includes a trichrome and mucin stain, and the second will include a
fungus
> and an acid fast stain. You will be asked to submit a control (for
special
> stains) stained with the method used in your laboratory. Also information
> will be collected from each laboratory on specimen preparation, reagents
> used, etc. A report will go back to the laboratory with a score on each
> slide, along with ratings of all other participating laboratories (not by
> name of course), and along with statistics on all demographic
information.
> One of the most important pieces of information to be returned will be
> educational material on each procedure (principle, what the pathologists
is
> looking for when he orders the stain, pitfalls, etc.) along with a
> photograph of a good stain. We also hope to send methods from the best
> performing laboratories, so that if needed, a participating laboratory
can
> use this to modifiy and improve their procedure. It is anticipated that
some
> time in the future, one of the special stains requested will be an
> immunohistochemical stain.
>
> This program will be limited to 300 participants the first year, and then
> will be opened to a larger number the second year.
>
> NSH is excited about the program, having worked on initiating an EQA
program
> for several years, and now having CAP as a co-sponsor. It is the first
> program designed specifically for the anatomic pathology laboratory, and
> should be a help when inspection time comes around. Those participating
in
> the pilot program found that the inspectors were pleased with the quality
> assessment documentation provided at that time, and this will be much
more
> extensive.
>
> Hope this helps.
>
> Freida Carson
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 15:00:41 -0500
> From: leish27@earthlink.net
> Subject: RE: Daily Digest
>
> Hello everyone. I was talking to some people at work and they told me some
> people still do histology at home. This sounds interesting to do on a
> part-time basis or something. Is this something that is gaining
popularity?
> Can anyone share any information on how this would be done or if they know
> someone that does it I can talk to. Thanks...just curious.
>
> - --- Felisha Sandifer
> - --- leish27@earthlink.net
> - --- EarthLink: The #1 provider of the Real Internet.
>
>
> > [Original Message]
> > From: HistoNet Server
> > To: HistoNet Server
> > Date: 10/7/2002 1:00:10 AM
> > Subject: Daily Digest
> >
> >
> > ----------------------------------------------------------------------
> >
> > Date: 6 Oct 2002 00:45:26 -0500
> > From: "Ms. Evelyn Kaplan"
> > Subject: Re: Soap
> >
> > Hi Marjorie,
> >
> > Taking it that this is not a set up.............
> > When I was a very raw junior, one of our laboratory jokes was to embed a
> > piece of soap in wax and give it to the newest recruit of the department
> to
> > try and cut a section. I was the next on the hit list and of course
when I
> > cut a lovely 3 microns section was very disappointed when it hit the
> water
> > bath and disappeared. However, I floated it out on alcohol and my senior
> > member of staff was amazed I had got any thing of the block! Let alone a
> > section you could see.
> > I am in the same position were the College of Engineering have asked me
> to
> > cut thin sections of black polythene waste pipes!
> > Give it a try..............
> >
> > Evelyn Kaplan
> > Dept of Pathology,
> > Sultan Qaboos University,
> > Muscat, Oman
> >
> >
> >
> > ----------------------------------------------------------------------
> >
> > Date: 6 Oct 2002 16:15:14 -0500
> > From: Cheryl Powell
> > Subject: National COnference
> >
> >
> >
Hi everybody! So how was the NSH national
meetings?
> > COUld people share something they learned new or improved for those of
us
> who
> > couldn't make it?
> >
Cheryl Ann Powell B.S.,
> > HTL(ASCP)
Botsford
> > General Hospital
> >
> >
Farmington Hills, MI
> > USA
> >
Chat with friends online, try MSN
> > Messenger: Click
> > Here
> >
> >
> > ----------------------------------------------------------------------
> >
> > Date: 6 Oct 2002 20:45:47 -0500
> > From: Michael & Victoria Franklin
> > Subject: HTL Exam
> >
> > Hello~
> >
> > I am studying to take the HTL exam and I was hoping for some input from
> > others who have taken it.
> >
> > If anyone could offer helpful info (subjects to pay particular attention
> > to, good texts and/or study guides, etc.), I would love to hear from
> > you!
> >
> > Many thanks,
> > Vicki
> >
> >
> >
> > Here are the messages received yesterday!
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 15:30:42 -0500
> From: Ryan Blair Rountree
> Subject: Calcein Blue and Tetracycline Bone Fluorescence
>
> I am trying to characterize the bone deposition rates in mouse feet. I
> have read several papers and plan to inject calcein blue first, wait 7
> days, inject tetracycline, wait 2 days, then plastic embed and section.
> However, I still have a few questions and any tips would be greatly
> appreciated.
>
> What dosage is best for calcein blue? I have seen it used at 32mg/kg, but
> this was for sacrificing the animals 6 hours later. I plan to sacrifice
> the animal 9 days later - should I use a lower dose?
>
> What concentration and in what (water?) do you make up the calcein blue
> and tet STOCK solutions? How do you store them?
>
> How careful do you have to be during the sectioning to prevent light
> exposure? Do you have to put the slides immediately into a dark box as
> you cut, or can you wait until the session is done?
>
> Will a filter usually used for detecting the DNA stain DAPI that lets
> 360-380nm light through excite tetracycline enough to visualize it? It's
> excitation peak is 390nm (range 380-420), and this borderline.
>
> Thanks for your help!
>
> Ryan
>
> Ryan Rountree
> Department of Developmental Biology
> Stanford, CA 94305
> Fax: (650) 725-7739
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 15:30:57 -0500
> From: Pamela.Neill@AlconLabs.com
> Subject: Meeting of the Texas Society for Microscopy Oct. 24-26
>
> The Fall 2002 TSM meeting will be held at Embassy Suites Austin North in
> Austin, Texas October 24-26. Workshops , cryo-microtomy with TEM sample
> preparation and TEM materials preparation tools, will be held during the
> meeting. There will be podium and poster sessions Friday and Saturday.
> Check out the website www.texasmicroscopy.org for more details.
>
> Pamela Neill
> TSM President 2002-2003
> www.texasmicroscopy.org
> 817-568-6497
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 15:45:10 -0500
> From: Cynthia Favara
> Subject: RE: lens of rat/mice
>
> Cynthia,
>
> I have done mouse eye including the lens in paraffin and frozen with good
> results. What information would you like to have.
>
> Cynthia Favara
> NIAID/NIH/RML/LPVD
> 903 South 4th Street
> Hamilton, MT 59840
> 406-363-9317
>
>
> - -----Original Message-----
> From: CYNTHIA HAYNES [mailto:cynthiahaynes@hotmail.com]
> Sent: Monday, October 07, 2002 12:44 PM
> To: histonet@pathology.swmed.edu
> Subject: lens of rat/mice
>
>
>
>
> Dear Histonetters,
>
> I would like to know if there is anyone out in H&E land who has
> experience with cutting frozen sections on rat and/or mice lens. I've
tried
> cutting them fresh and fixed and both have left with shattered lens.
Please
> e-mail me with any information on how to section them correctly.
> Sincerely yours,
> Cynthia Haynes
>
>
> P.S. My e-mail address is cynthiahaynes@hotmail.com
>
> _________________________________________________________________
> Join the worldis largest e-mail service with MSN Hotmail.
> http://www.hotmail.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 16:15:12 -0500
> From: Instrumedics
> Subject: NSH meeting
>
> Dear Histonetters,
> We want to thank all of you who visited our booth for a demonstration of
the
> CryoJane Tape-Transfer technology.
>
> Please let us know if you need any further information. We can send you a
> video that is narrated by Dr. Richard Zarbo, of the Henry Ford Hospital,
> that has a full demonstration of the CryoJane process, from snap freezing
> the tissue to fixation.
>
> This offer is open to all!
>
> Thanks again.
>
> Bernice
> schiller@instrumedics.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 17:16:06 -0500
> From: Robert Brunner
> Subject: RE: HT/HTL tissue list
>
>
> Melissa
>
> The tissue lists will probably be out around the 21st of October.
>
> Robert Brunner
> Program Director
> Argosy University
> Histology Technician Program
>
> - -----Original Message-----
> From: Melissa Jans [mailto:histomjans@yahoo.com]
> Sent: Monday, October 07, 2002 1:14 PM
> To: histonet@pathology.swmed.edu
> Subject: HT/HTL tissue list
>
>
>
> Does anyone know when the list of tissues will be mailed out for the
HT/HTL
> exam applications that were due on October 1st. We have some anxious
people
> in the lab who want to get started!
>
> Thanks,
>
> Melissa Jans
>
>
>
>
>
>
> _____
>
> Do you Yahoo!?
> Faith Hill - Exclusive Performances, Videos, &
more
> faith.yahoo.com
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_+JwSB8hh/rFsuFofgC0WcA)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_+JwSB8hh/rFsuFofgC0WcA)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> size=2>Melissa
> size=2>
>
> The
> tissue lists will probably be out around the 21st of October.
>
> size=2>
> size=2>Robert
> Brunner
> size=2>Program Director
> size=2>Argosy
> University
> size=2>Histology Technician Program
>
>
> Does anyone know when the list of tissues will be mailed out for the
> HT/HTL
> exam applications that were due on October 1st. We have some
> anxious people in the lab who want to get started!
> Thanks,
> Melissa Jans
>
>
>
> Do you Yahoo!?
Faith Hill -
> Exclusive
> Performances, Videos, & more
href="http://faith.yahoo.com">faith.yahoo.com
>
>
> - --Boundary_(ID_+JwSB8hh/rFsuFofgC0WcA)--
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 19:30:24 -0500
> From: Lee & Peggy Wenk
> Subject: Re: HT/HTL tissue list
>
>
> For those who would like to start collecting the registry practical
tissues
> early - go to the following web site of ASCP Board of Registry:
>
> http://www.ascp.org/bor/certification/procedures
>
> Then either click on Histologic Technician or Histotechnologists,
depending if
> you are planning on taking the HT or HTL exam. This will get you to a page
> (for each) that lists the routes. Scroll to the bottom of the routes
page. At
> the bottom, is a link to the practical exam (for each).
>
> On the practical exam list is ALL the possible tissues they might ask you
to
> collect, and ALL the possible stains on each tissues, that they might ask
for.
> For the HTL, that's 28 tissues, with between 1 and 6 stains for each
tissue.
> For the HT, that's 25 tissues, with between 1 and 5 stains for each
tissue.
>
> So the 9 tissues/stains the BOR will ask for each practical will come off
> these lists - HT and HTL.
>
> For my students, we start collecting tissues months BEFORE they ever
receive
> their exam lists. We use these web site lists to collect the tissues, get
the
> correct components, gross them the right dimensions, etc. We process the
> tissue, embed them, and cut and stain and do an H&E. We then look at the
H&E.
> If the tissue/block is good, we hang onto it, just in case the BOR asks
for
> that tissue. If the tissue isn't good enough (too small, slightly
autolyzed,
> wrong part of the organ, etc), we know to go looking for better.
>
> By collecting early off of this list, then once the "official" exam list
> comes, we already have a good head start on the collection of tissues. And
> sometimes, finding a colon or gallbladder with columnar epithelium across
one
> entire surface can be surprisingly difficult. Or finding an ovary or
tonsil
> that's 1.5 x 1.5 cm square can also be difficult, as it needs to be this
size
> so that it can shrink in processing yet remains bigger than the 1.0 x 1.0
cm
> square requirement.
>
> Each year, we have one tissue that gives us problems. Stomach one year,
> esophagus another, gall bladder another. It was liver last year. And each
> year, it's a different tissue that seems to give us difficulties. We will
just
> have problem getting a good, non-autolyzed, well fixed, epithelium all
intact,
> tissue on one tissue. And that's with starting months in advance.
>
> So my advise is . . . collect early, collect often, collect a lot.
>
> Hope that helps.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>
> ----- Original Message -----
> From: Melissa Jans
> To: histonet@pathology.swmed.edu
> Sent: Monday, October 07, 2002 2:13 PM
> Subject: HT/HTL tissue list
>
>
> Does anyone know when the list of tissues will be mailed out for the
HT/HTL
> exam applications that were due on October 1st. We have some anxious
people
> in the lab who want to get started!
>
> Thanks,
>
> Melissa Jans
>
>
>
>
>
>
>
> -
>
----------------------------------------------------------------------------
--
> Do you Yahoo!?
> Faith Hill - Exclusive Performances, Videos, & more
> faith.yahoo.com
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_Y9tPqDCSVjm07OMIe5Qp/Q)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_Y9tPqDCSVjm07OMIe5Qp/Q)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> For those who would like to start collecting
the
> registry practical tissues early - go to the following web site of ASCP
Board
> of
> Registry:
>
>
>
> Then either click on Histologic Technician
or
> Histotechnologists, depending if you are planning on taking the HT or HTL
> exam.
> This will get you to a page (for each) that lists the routes. Scroll to
the
> bottom of the routes page. At the bottom, is a link to the practical exam
(for
>
> each).
>
> On the practical exam list is ALL the
possible
> tissues they might ask you to collect, and ALL the possible stains on
each
> tissues, that they might ask for. For the HTL, that's 28 tissues, with
between
> 1
> and 6 stains for each tissue. For the HT, that's 25 tissues, with between
1
> and
> 5 stains for each tissue.
>
> So the 9 tissues/stains the BOR will ask for
each
>
> practical will come off these lists - HT and HTL.
>
> For my students, we start collecting tissues
> months
> BEFORE they ever receive their exam lists. We use these web
site lists to
>
> collect the tissues, get the correct components, gross them the right
> dimensions, etc. We process the tissue, embed them, and cut and stain and
do
> an
> H&E. We then look at the H&E. If the tissue/block is good, we hang
> onto
> it, just in case the BOR asks for that tissue. If the tissue isn't good
enough
>
> (too small, slightly autolyzed, wrong part of the organ, etc), we know to
go
> looking for better.
>
> By collecting early off of this list, then
once
> the
> "official" exam list comes, we already have a good head start on the
> collection
> of tissues. And sometimes, finding a colon or gallbladder with columnar
> epithelium across one entire surface can be surprisingly difficult. Or
finding
>
> an ovary or tonsil that's 1.5 x 1.5 cm square can also be difficult, as it
> needs
> to be this size so that it can shrink in processing yet remains
bigger
> than
> the 1.0 x 1.0 cm square requirement.
>
> Each year, we have one tissue that gives us
> problems. Stomach one year, esophagus another, gall bladder another. It
was
> liver last year. And each year, it's a different tissue that seems to
give us
> difficulties. We will just have problem getting a good,
non-autolyzed,
> well
> fixed, epithelium all intact, tissue on one tissue. And that's with
starting
> months in advance.
>
> So my advise is . . . collect early,
collect
>
> often, collect a lot.
>
> Hope that helps.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>
> style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px;
BORDER-LEFT:
> #000000 2px solid; MARGIN-RIGHT: 0px">
> ----- Original Message -----
> style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color:
> black">
From:
>
Melissa
> Jans Sent: Monday, October 07, 2002
2:13
> PM
> Subject: HT/HTL tissue list
>
> Does anyone know when the list of tissues will be mailed out for the
> HT/HTL
> exam applications that were due on October 1st. We have some
> anxious people in the lab who want to get started!
> Thanks,
> Melissa Jans
>
>
>
> Do you Yahoo!?
Faith Hill -
> Exclusive
> Performances, Videos, & more
href="http://faith.yahoo.com">faith.yahoo.com
>
>
> - --Boundary_(ID_Y9tPqDCSVjm07OMIe5Qp/Q)--
>
>
> ----------------------------------------------------------------------
>
> Date: 7 Oct 2002 19:30:39 -0500
> From: Melissa Jensen
> Subject: Re: written portion of HT
>
> CONGRATS!!!!!!!! WELL DONE!
> - ----- Original Message -----
> From:
> To:
> Sent: Saturday, October 05, 2002 1:22 PM
> Subject: Re: written portion of HT
>
>
> > I was the one last week that thought I bombed the written portion of the
> HT. I passed!!!!! Just had to let you know cause Im elated!!! Thanks Jill
> >
>
>
>
> Here are the messages received yesterday!
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