RE: Connexin immunohistochemistry

From:"Georger, Mary"

John,
I have done beautiful Connexin 43 on FFPE mouse hearts, would be happy to
share the protocol. 
 <<...OLE_Obj...>> 
If you have any questions give me a shout, best of luck!
Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	J. A. Kiernan
> Sent: 	Tuesday, October 15, 2002 12:13 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Connexin immunohistochemistry
> 
> One of our graduate students wants to immunostain
> for connexin 43 in paraffin sections of rats' hearts.
> No luck so far, using a rabbit primary and a
> Texas red-labelled goat anti(rabbit) secondary. 
> Does anyone know if these gap junction proteins 
> can be immunostained after paraffin embedding? 
> (Most of the gapjunctologists round here study 
> cultured cells.)
>                  John Kiernan
> -- 
> -------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London,   Canada   N6A 5C1
>    kiernan@uwo.ca
>    http://publish.uwo.ca/~jkiernan/
> 
> 



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