RE: Connexin immunohistochemistry
I have done beautiful Connexin 43 on FFPE mouse hearts, would be happy to
share the protocol.
If you have any questions give me a shout, best of luck!
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York 14642
> From: J. A. Kiernan
> Sent: Tuesday, October 15, 2002 12:13 PM
> To: firstname.lastname@example.org
> Subject: Connexin immunohistochemistry
> One of our graduate students wants to immunostain
> for connexin 43 in paraffin sections of rats' hearts.
> No luck so far, using a rabbit primary and a
> Texas red-labelled goat anti(rabbit) secondary.
> Does anyone know if these gap junction proteins
> can be immunostained after paraffin embedding?
> (Most of the gapjunctologists round here study
> cultured cells.)
> John Kiernan
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
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