RE: Chloroacetate Esterase

From:"DeLovino, Salvacion S."

In our old procedure, there was a comment section that says esterase is
inhibited to varying degrees by mercury, acid solutions, heat, and iodine.

Salvacion Delovino
> ----------
> From: 	Johnston, Kathy[]
> Sent: 	Friday, October 11, 2002 8:39 AM
> To: 	Histonet (E-mail)
> Subject: 	Chloroacetate Esterase
> We are using Sigma's Chloroacetate Esterase kit, and are doing one on a B5
> fixed bone marrow slide.  Our problem is thus:  Both myself and the stains
> tech recall in the recesses of our collective memories, that Na
> thio/iodine
> to remove the mercury pigment produces a false negative for this test.
> Is there anyone else out there that confirms or contradicts this??  We are
> reluctant to proceed and possibly ruin a slide, as well, we do not want to
> stain it, and leave a bunch of mercury which may impair diagnosis.
> Your help is greatly appreciated!
> Kathy Johnston
> Tech II - Special Stains
> Anatomic Pathology - Foothills Medical Center
> Calgary Laboratory Services
> Ph - 403-944-4760
> Fax - 403-270-4093

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