We currently have the antibody worked up to be done on our DAKO Autostainer.
It does have to be done on frozen sections.  If you would like a copy of our
protocol let me know and I will either e-mail or fax it to you.

Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth
817-878-5698 fax
817-878-5644 office
donnawillis@texashealth.org

-----Original Message-----
From: Klassen, Shannon SDH [mailto:klassens@sdh.sk.ca]
Sent: Tuesday, October 08, 2002 10:16 AM
To: 'Martha Ward'
Cc: 'Histonet'
Subject: RE: C4d staining for renal biopsies


I would be interested in any replies also.
Shannon Klassen
IHC Tech
Saskatoon Health District
Saskatoon, Canada

> ----------
> From: 	Martha Ward[SMTP:mward@wfubmc.edu]
> Sent: 	Friday, September 27, 2002 9:22 AM
> To: 	HISTONET@pathology.swmed.edu
> Subject: 	C4d staining for renal biopsies
> 
> Our lab has had inquiries about adding this antibody to our protocol for
> transplant renal biopsies and I wanted to hear from labs that are
> currently performing it.  All the protocols I have seen reference frozen
> tissue but we were wondering if there is a way to do it on ffpe tissues.
> Any and all help will be greatly appreciated!
>  
> Martha Ward, MT (ASCP) QIHC
> Wake Forest University Baptist Medical Center
> Winston-Salem, NC
> 1-336-716-2756
>