RE: A dye by any name would stain as red?
Our Neuro lab is using Solvent Blue 38 from Sigma, Cat# S-3382 for their LFB
stain. Have been for a long time now, and they work beautifully. Hope this
can help your predicament!
From: R.Z. Meitus [mailto:email@example.com]
Sent: Wednesday, October 09, 2002 1:30 AM
To: Bruce Gapinski; 'HistoNet@Pathology.swmed.edu'
Subject: Re: A dye by any name would stain as red?
I sort of understand your predicament but it is with
Luxol Fast Blue.
LFB-PAS is not gonna happen with this stuff...LFB
(same C.A.S. as the old stuff, but WAY more purple;
ordered from A*** As*** as that was the most readily
available ...disappears during lithium carbonate
with the collaberation of colleagues we have surmised
that you can't get (the right) LFB anymore...it is
worth more than gold around here...no one can get the
--- Bruce Gapinski wrote:
> Does anyone else still make their own Kinyoun's
> Carbol Fuchsin Solution for
> the AFB stain? If so, maybe you can help me. When
> you reorder Basic Fuchsin
> do you go by the C.I. # (color index) or the
> catalogue #? I was taught to go
> by CI #. It's been many years since I last ordered
> and my bottle had the cat
> # B660-3 CI # 42500. So I pulled my order card and
> more dye from JT *****,
> through V**. The bottle I received was named Basic
> Fuchsin Hydrochloride. OK
> the old bottle didn't have "Hydrochloride" on the
> label but what's more is
> that the CI number was wrong! It was Cat # B660-03
> (right #) and CI #
> 42510(wrong CI#) I called V** and asked for a bottle
> with the correct CI
> number (42500). I got it. Its name is Pararosanilin
> Hydrochloride CI 42500!
> No mention of Basic Fuchsin anywhere on the label.
> Just looked it up in
> AFIP, Ann Preece, and F.Carson's books- no mention
> of CI number. Which dye
> would you use? Or am I'm going to have to do some QA
> with these two dyes?
> Bruce Gapinski
Rebecca Z. Meitus, HTL
Vanderbilt University Medical Center
Department of Pathology
Division of Neuropathology
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