From: | Cathy Mayton |
Dissolve 0.365 g of Toluidine Blue in 50 ml of 30%
ethanol
then add 0.135 g of Basic Fuchsine and stir until dissolved.
I have seen some variations call for the addition of
0.75 g of sodium tetraborate but
this is not in the original recipe.
I believe the original references are:
Martin et
al., Am. J. Clin Path. 46:250-251, 1966.
Spurlock et al., Am. J. Clin. Path.
46:252-258, 1966.
That's right, the papers are back to back in the same
journal.
2) I worked with Martin et al. and the sodium
borate was added to the Paragon
stain to help the penetration into epon
embedded sections. The Paragon stain
was designed for frozen sections
and was marketed by a company by the name of
Paragon. The formula that
appeared on the bottles of Paragon stain was as
follows:
Toluidine
blue CI 520040 ................0.73 gm
Basic fuchsin , CI 42510
................0.27 g,
Ethyl alcohol, 30% ........................100
ml
If adding sodium borate, it must not be added to the bottle, as it
affects
the stability, but is added to the stain on the slide - only a
little pinch.
3) I notice that the color index for the Basic
Fuchsin in this formula is
42510. Would that be the same color index for
Basic Fuchsin in Kinyoun's AFB
stain?
4) A quick Internet search found the following:
http://www.histosearch.com/histonet/Jun00A/Re.Paragonstain.html
5) I am not sure that it is still manufactured, but I have my own
version if you are interested.