High Iron Diamine stain

From:"Martin, Ronald"

Fellow Techs,
I have a staining question regarding the fore mentioned stain. My question
is about the ferric chloride needed for this stain. The only information for
a procedure I could find states "ferric chloride (60% BDH solution)-1.4
ml's". I have anhydrous ferric chloride but how should I make up a 60%
solution? The MSDS sheet states not to expose to water or moist air because
of a possible explosive situation and an exothermic reaction. Should I use
an alcohol solution with the anhydrous ferric chloride? I know from
experience that ferric chloride and water does cause a very exothermic
If anyone has experience with this stain, I would appreciate some guidance.
Thanks in advance,
Ron Martin
Research Associate

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