Hi Histonetters,

I am posting this for a co-worker who does not receive histonet but needs 
some help. Please respond directly to her. Thanks.






>Question:
>
>
>Has anyone had any experiences with the non-specific  immunostaining of
>eosinophils?  Our tissue is fixed in Bouin's fixative, embedded in
>paraffin, immunostained using the Peroxidase-Anitperoxidase immunotechnique
>and visualized for light microscopy with DAB.   We do not think the
>antibody should be reacting with antigen in the eosinophils, but that is
>the structure that stains really well.  It has happened with both a rabbit
>polyclonal and mouse monoclonal antibody.    If anyone else has experienced
>this, please e-mail me at skperkin@vt.edu.
>
>Thanks so much!!
>
>Sandy Hancock
>
>
>
>
>
>
>"If a man does not keep pace with his companions, perhaps it is because he
>hears a different drummer...."
>                                                         - Henry David Thoreau


**************************************************************************
Jill Songer HT (ASCP)
Histopathology Lab
Virginia Maryland Regional College
of Veterinary Medicine
Veterinary Teaching Hospital
Blacksburg, VA 24061