FW: Chloroacetate Esterase

From:"Johnston, Kathy"

I do apologize for the rough language, but I've had just about enough from
this person.  Is there any possible way of preventing them from accessing
Histonet in the future?

-----Original Message-----
From: rene [mailto:rene-vh@planet.nl]
Sent: Friday, October 11, 2002 11:10 AM
To: Johnston, Kathy
Subject: Re: Chloroacetate Esterase


----- Original Message -----
From: Johnston, Kathy 
To: Histonet (E-mail) 
Sent: Friday, October 11, 2002 5:39 PM
Subject: Chloroacetate Esterase

> We are using Sigma's Chloroacetate Esterase kit, and are doing one on a B5
> fixed bone marrow slide.  Our problem is thus:  Both myself and the stains
> tech recall in the recesses of our collective memories, that Na
> to remove the mercury pigment produces a false negative for this test.
> Is there anyone else out there that confirms or contradicts this??  We are
> reluctant to proceed and possibly ruin a slide, as well, we do not want to
> stain it, and leave a bunch of mercury which may impair diagnosis.
> Your help is greatly appreciated!
> Kathy Johnston
> Tech II - Special Stains
> Anatomic Pathology - Foothills Medical Center
> Calgary Laboratory Services
> Ph - 403-944-4760
> Fax - 403-270-4093
> kathy.johnston@cls.ab.ca

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