ECM Autofluorescence blues - any solutions?
Thanks to everyone for the help and suggestions regarding what I mistakenly
assumed was non-specific binding of my secondary antibody. Now I find I
have a different problem altogether...
The extracellular matrix (ECM) laid down by my human smooth muscle cells
fluoresces red at 594nm (so fluorescently staining with an antibody that
fluoresces red is pointless) and which also fluoresces green at 495nm (so
using a green fluorescing antibody is no good either).
I've tried using 100mM - 500mM Glycine, which worked for Andrea who kindly
responded to my plea last time I was in touch.
Does this mean that fluorescently staining adsorbed ECM is not possible or
is someone out there doing it?
Any suggestions gratefully received,
Department of Clinical Engineering,
The University of Liverpool,
Tel: +44(0)151 706 5198
Fax: +44(0)151 706 5803
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