Connexin immunohistochemistry

From:"J. A. Kiernan"

One of our graduate students wants to immunostain
for connexin 43 in paraffin sections of rats' hearts.
No luck so far, using a rabbit primary and a
Texas red-labelled goat anti(rabbit) secondary. 
Does anyone know if these gap junction proteins 
can be immunostained after paraffin embedding? 
(Most of the gapjunctologists round here study 
cultured cells.)
                 John Kiernan
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/



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