Connexin immunohistochemistry

From:"J. A. Kiernan"

One of our graduate students wants to immunostain
for connexin 43 in paraffin sections of rats' hearts.
No luck so far, using a rabbit primary and a
Texas red-labelled goat anti(rabbit) secondary. 
Does anyone know if these gap junction proteins 
can be immunostained after paraffin embedding? 
(Most of the gapjunctologists round here study 
cultured cells.)
                 John Kiernan
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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