Alk Phos osteoblast staining

From:Ryan Blair Rountree

Can anyone send me a protocol for staining osteoblasts with Alkaline
Phosphatase?  I am planning to do this on fixed frozen tissue that is from
young enough mice that I can still section tissue just beginning to
ossify.  Is it as simple as just sectioning the tissue and incubating it
in NBT/BCIP?  Or are there other complexities?

Thanks for any input!!


Ryan Rountree
Department of Developmental Biology
B301, Beckman center
279 Campus Drive
Stanford, CA 94305
(650) 725-7599
Fax: (650) 725-7739

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