Alk Phos osteoblast staining
Can anyone send me a protocol for staining osteoblasts with Alkaline
Phosphatase? I am planning to do this on fixed frozen tissue that is from
young enough mice that I can still section tissue just beginning to
ossify. Is it as simple as just sectioning the tissue and incubating it
in NBT/BCIP? Or are there other complexities?
Thanks for any input!!
Department of Developmental Biology
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