Greetings,

I am trying to use Thionin to stain for chromatin clumps associated with
apoptosis in fetal monkey brain.  I seem to be getting nice blue
staining of the smaller support cells, but weak pink/purple staining of
neurons.  The tissue has already undergone immunostaining.  I have
altered the pH of the Thionin solution ranging from 3.5 to 11 and still
can't seem to get selective neuronal staining.  I have used nissl stains
in fetal and adult rat tissue without any problems, and am kinda stuck
on this one.  Ideas, anyone?

Thanks in advance
____________________________________________
Bret A. Morrow, Ph.D.
Associate research scientist
Associate clinical professor
Yale University School of Medicine
New Haven, CT 06520-8066

voice 203.785.4515
fax  203.785.7670
email  bret.morrow@yale.edu