If you would like to demonstrate the spores in the bacillus you can use the
Schaeffer-Fulton spore staine.


Jennifer MacDonald


----- Original Message -----
From: "Morken, Tim" 
To: "'Histonet'" 
Sent: Wednesday, October 24, 2001 9:16 AM
Subject: more on anthrax special stains


> I neglegted to mention we also use the Steiner & Steiner silver stain,
which
> is more reliable than a gram, but also more difficult to interpret.
>
> -----Original Message-----
> From: Morken, Tim
> Sent: Wednesday, October 24, 2001 12:15 PM
> To: 'Hoye, Glenda F. (Fka Hood)'; 'Histonet@pathology.swmed.edu'
> Subject: RE: anthrax
>
>
> We use a gram stain (anthrax is gram+), although in tissue from this event
> staining has been variable. For specific detection we cell wall and cell
> capsule antibodies developed by the CDC and the US Army Research Institute
> for Infectious Disease (USAMRIID). As far as I know these antibodies are
not
> availble commercially.
>
> Tim Morken
> CDC, Atlanta
>
> -----Original Message-----
> From: Hoye, Glenda F. (Fka Hood) [mailto:ghoye@iupui.edu]
> Sent: Wednesday, October 24, 2001 10:38 AM
> To: 'Histonet@pathology.swmed.edu'
> Subject: anthrax
>
>
> Forgive me if this topic has already been discussed 'ad nauseum' when I
was
> logged off histonet last week, but I was wondering if there are specific
> histologic stains to detect the anthrax bacillus in tissues? The pictures
> they show on TV look like gram stains, but I'm sure those may be smears.
Are
> the Gram modifications done in histo the most effective ones at
> demonstrating it in tissue? Or is this an organism that isn't readily
> demonstrated in tissue? Any discussion, OR direction to the archives to
look
> at previous discussions, would be appreciated.
>
> Thanks,
> Glenda Hoye
>
>
>