Dear Andi,

I have successfully used 3-5% glycerol for this purpose, on skin sections.
Thirty minutes was usually adequate. You don't mention the tissue type or
section thickness which could be factors as well. How are you storing your
slides and which fixative do you use ? Are your slides coated with adhesive
or charged ? Are all the sections affected ?
I am not a guru but I understand some of problems.

hooroo,richard
-----Original Message-----
From: Andrea Grantham 
To: histonet@pathology.swmed.edu 
Date: Thursday, 25 October 2001 17:33
Subject: ? for laser capture gurus


>I have run into a problem with some frozen sections I did for laser capture
>microdissection and I'm wondering if anything can be done to salvage the
>slides that I cut (many slides!).
>When I did the frozens I picked up the tissue on my usual slides which are
>the Snowcoat X-tra slides from Surgipath. Now that the tissue is "nailed"
>to the glass it won't come loose and allow itself to be captured. Is there
>anything that can be done or is it back to the cryostat?
>Thanks!!
>Andi Grantham
>.....................................................................
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
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