Re: antigen retrieval

From:JOE NOCITO

Julie,
if the doctors ordered immunos on a smear, cytospin or thinprep, we just fix
them in alcohol and run them without any pretreatment.  The only time we did
a pretreatment was on the cell block.  Hope this helps.

Joe Nocito, BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, Texas
----- Original Message -----
From: "Julie Collins" 
To: 
Sent: Tuesday, October 23, 2001 12:28 PM
Subject: antigen retrieval


I was wondering how many people (if any) are doing antigen retrieval on
their cytology specimens prior to immunostaining?  If so, could you share
your protocols?

Thanks,
Julie Collins
Dir, Immunohistochemistry
Univ. of Kansas Med. Center
Kansas City, KS







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