Julie,
If the smears have been alcohol fixed or air dried,
then there is no need to retrieve. Retrieval, so I am
led to believe, reverses the effect of formalin
fixation to some degree. Since cytology smears are
usually not formalin fixed, then retrieval is not
required. Just pop the localisation antibody straight
on. Beware that some antigens may be affected by
alcohol.
 



--- Julie Collins  wrote: > I was
wondering how many people (if any) are doing
> antigen retrieval on their cytology specimens prior
> to immunostaining?  If so, could you share your
> protocols?
> 
> Thanks,
> Julie Collins
> Dir, Immunohistochemistry
> Univ. of Kansas Med. Center
> Kansas City, KS
> 
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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