At 10:16 AM 10/24/01 -0600, Gayle Callis wrote:
>It is gram positive rods, but not having done actual infected tissue, don't
>know how hard it is to find bacillus.  One microbiologist here showed a
>photo of bacillus with spores, stained with a polychrome methylene blue -
>wonderful staining of the spores.  

Did this actually stain the endospores???  usually, these are impervious to
dyes and apear as clear, refractile bodies within the vegetative cell.
however, the only  method for staining them than I know of  uses malchite
green.  This sounds really cool, Gayle.  I'd love to see it... wish I could. 

Connie M.

>
>Check out some microbiology texts and pathology books describing
>pathogenesis of disease, maybe these will have some photos of what you want
>to see.  Let us know what you find, it would be educational for all of us.
>Hopefuly our CDC Histonetters will lead us in the right direction.
>
>  
>Gayle Callis
>MT,HT,HTL(ASCP)
>Histopathology Supervisor
>Veterinary Molecular Biology - Marsh Lab
>Montana State University - Bozeman
>19th and Lincoln St
>Bozeman MT 59717-3610
>
>406 994-6367
>406 994-4303 (FAX)
>
>
>

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