Well.
if I didn't see it with my own eyes, I would have never believed it.  We ran
side-by-side samples, four on positve -charged slides and four on freshly
treated poly-l-lysine slides. The staining and quality on the
positive-charged slides were poor.  Staining was uneven and cells floated
off.  The reason why is beleived to be in the manufacturing of the
positive-charged slides. With the poly-l-lysine treated slides, the slides
are dipped in the solution, then stood on end to air dry.  It is recommended
that you only prepare the number of slides you need for that run.  It seems
dust and other debris reek havoc when using the Autocyte stainer.
    My question is this, "are we supposed to be moving forward in
technology?"  I stopped using poly-l lysine treated slides years ago.  I'm
getting confused.

Joe Nocito, BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, Texas
----- Original Message -----
From: "Dan Diaz" 
To: ; 
Sent: Friday, October 26, 2001 12:32 PM
Subject: Re: TriPath Autocyte machine users


>
> Im confused, I thought Poly - L were ++ charged slides?  If not then what
is
> the difference and also what are Amino Alco Saline Slides?  Positive
Charged
> or no?
>
> >From: JOE NOCITO 
> >To: HistoNet Server 
> >Subject: TriPath Autocyte machine users
> >Date: Thu, 18 Oct 2001 17:49:45 -0700
> >
> >Hello all,
> >I had a technician  return from the training session at Tripath in N.C.
> >She
> >informed me that we have to use poly-l-lysine treated slides for the
cells
> >to stay on.  I asked her about using positive charged slides.  She said
> >they
> >told her it wouldn't work.  I told that was (never mind, I won't write
what
> >I said) but I stopped using poly-l-lysine slides years ago.
> >     My question.  Does anyone use the positive charged slides on the
> >Autocyte?  Are you having problems with the cells falling off? Since the
> >machine also stains the slides,is there background staining on the
slides?
> >I think this is all hogwash, but I've been wrong before.  Thanks again
> >
> >Joe Nocito, BS, HT (ASCP) QIHC
> >Histology Manager
> >Pathology Reference Lab
> >San Antonio, Texas
> >
> >
>
>
> _________________________________________________________________
> Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
>
>