Im confused, I thought Poly - L were ++ charged slides?  If not then what is 
the difference and also what are Amino Alco Saline Slides?  Positive Charged 
or no?

>From: JOE NOCITO 
>To: HistoNet Server 
>Subject: TriPath Autocyte machine users
>Date: Thu, 18 Oct 2001 17:49:45 -0700
>
>Hello all,
>I had a technician  return from the training session at Tripath in N.C.  
>She
>informed me that we have to use poly-l-lysine treated slides for the cells
>to stay on.  I asked her about using positive charged slides.  She said 
>they
>told her it wouldn't work.  I told that was (never mind, I won't write what
>I said) but I stopped using poly-l-lysine slides years ago.
>     My question.  Does anyone use the positive charged slides on the
>Autocyte?  Are you having problems with the cells falling off? Since the
>machine also stains the slides,is there background staining on the slides?
>I think this is all hogwash, but I've been wrong before.  Thanks again
>
>Joe Nocito, BS, HT (ASCP) QIHC
>Histology Manager
>Pathology Reference Lab
>San Antonio, Texas
>
>


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