Re: Schaeffer-Fulton Stain for bacterial spores

From:RSRICHMOND@aol.com

The Schaeffer-Fulton stain for bacterial spores - possibly useful in 
identifying anthrax bacilli - is indeed cited in H.J. Conn's Biological 
Stains, 9th ed. by R.D. Lillie 1977 - of which Richard Horobin just told me 
that he and John Kiernan are preparing a badly needed revision -

It was one of the stains that the Biological Stain Commission used to certify 
batches of malachite green (p. 579). Flame-fixed smears are flooded with 5% 
aqueous malachite green for about a minute, "then heated to steaming 3 or 4 
times". They are washed in water 30 sec, and 0.5% aqueous safranin is added 
for 30 seconds - wash and blot dry. Spores are green, the rest of the cell 
red. Nothing is said about anthrax bacilli.

The original reference is not given, but a reference to Conklin's 
modification replacing safranin with mercurochrome (a hazardous material 
that's been discussed in this forum repeatedly), and the original reference 
could probably be found in it.

Conklin ME Mercurochrome as a bacteriological stain. J Bacteriol 1934;27:30-31

This seems to me to be ancient history, unless someone can find an update of 
it.

Bob Richmond
Samurai Pathologist
Knoxville TN




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