Re: Schaeffer-Fulton Stain for bacterial spores
The Schaeffer-Fulton stain for bacterial spores - possibly useful in
identifying anthrax bacilli - is indeed cited in H.J. Conn's Biological
Stains, 9th ed. by R.D. Lillie 1977 - of which Richard Horobin just told me
that he and John Kiernan are preparing a badly needed revision -
It was one of the stains that the Biological Stain Commission used to certify
batches of malachite green (p. 579). Flame-fixed smears are flooded with 5%
aqueous malachite green for about a minute, "then heated to steaming 3 or 4
times". They are washed in water 30 sec, and 0.5% aqueous safranin is added
for 30 seconds - wash and blot dry. Spores are green, the rest of the cell
red. Nothing is said about anthrax bacilli.
The original reference is not given, but a reference to Conklin's
modification replacing safranin with mercurochrome (a hazardous material
that's been discussed in this forum repeatedly), and the original reference
could probably be found in it.
Conklin ME Mercurochrome as a bacteriological stain. J Bacteriol 1934;27:30-31
This seems to me to be ancient history, unless someone can find an update of
it.
Bob Richmond
Samurai Pathologist
Knoxville TN
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