Re: Periodic acid methanamine silver staining question
basement membrane in the glomeruli stains last. Usually only
takes 40 minutes in a 60 degree oven. We use a microwave to hasten
the silver staining. 1200 watts 50 percent power 40 seconds. check the
slides when they are a lt tan in color check microscopically for
end point, keep the silver warm by reheating for 20 seconds in the
microwave. takes from 1-4 minutes to stain. reference in Sam
Spicer's Histochemistry in Pathologic diagnosis
Rena Fail
At 02:56 PM 10/18/01 -0500, you wrote:
Hi all-
I am having a problem getting the basement membranes in the glomeruli
to
stain. The basement membranes of tubules and reticular fibers are
staining
very well. I'm using a kit from Polyscientific, Inc that
recommends
incubation in at 70C for 60-75 minutes. I've gone out to 90+
minutes with
no improvement. I was hoping somebody out there would be able to
shed some
light on the subject. I'm going to another protocol that calls
for
microwaving at 95C for 15 to 20 minutes but I wanted to see if there
were
any other thoughts out there.
Thanks for the help.
Dave
david.rosolia@aventis.com
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