Re: OG6/EA50 combination
Combining the OG-6 and EA-50 in the Pap stain has been considered Good
Management ever since I started doing pathology in the 1960's. Many
commercial staining systems - I could mention one - incorporate this
Theoretically, it shouldn't work - the Papanicolaou cytology stain is a
modified trichrome stain - read R.D. Lillie on the theory.
Practically speaking, it doesn't work - it's the first thing I look for when
the Pap stain is unreadable in a laboratory.
The Pap stain is infinitely capricious and changeable, and requires constant
interaction between the cytopathologist, the cytotechnologist, and the
cytopreparatory technologist. Too often I find somebody doing the cytology
staining who has never looked down a microscope in her life, with the
cytotechnologist threatened with job termination if she interferes.
The OG-6 (Orange G) stains keratin in the smear, while the EA (Light Green SF
or Fast Green FCF) is respnsible for the green cytoplasmic staining of
squamous cells before they keratinize. It is very difficult to interpret
cytologic preparations when this stain is inadequately balanced, and it is
the job of the cytotechnologist to see this done right.
<< Previous Message | Next Message >>