Found one for eosinophils. Is that what you are looking for?

Most of the web site procedures I found were for nasal swabs
or urine spins. Most just said to by the Hansel stain solution
from a vendor and follow directions from vendor. But I couldn't
find a vendor name.

Did find a procedure at the Allergy Society of South Africa
web site:

http://www.allergysa.org/html/nasal.html

I've tried to copy it below, but it looks MUCH better on the web page.

Hansel's Stain
        Method used referenced from Sheldon, Lovell and Mathews' "A Manual
of
            Clinical Allergy" (published by W B Saunders Company, 1967).

         1.  1:200 eosin stain
            0.30 g eosin
            60.00 ml methyl alcohol

         2. 1:100 Methylene blue stain
              0.60 methylene blue
             60.00 ml methyl alcohol

         3. Distilled water

         4. 95% ethanol


         Stains should be kept in amber dropper bottles and freshly made up
every 2 months.


        Staining Methods

           1.Flood an air dried slide with eosin stain and allow to stand
for
             1 minute.
           2.Add an equal volume distilled water for 1 minute.
           3.Drain and flood slide with distilled water until all the stain
is
             removed.
           4.Flood slide with 95% ethanol and then drain.
           5.Stain immediately with methylene blue for 1 minute.
           6.Add an equal volume distilled water until all the stain is
             removed.
           7.Flood slide with distilled water until all the stain is
removed.
           8.Flood slide with 95% ethanol and air dry.
           9.Examine under oil immersion.

Technique
           1.Small child to sit on mother's lap.
           2.Explain the procedure to the mother and child.
           3.Using a clean, dry glass rod gently remove mucus from inner
             surface of nostril.
           4.Spread evenly over surface of clean glass slide.
           5.Label and date slide.

        NOTE:
        An alternative way of collecting a sample would be for the patient
to
        blow their nose on wax paper.
       Nasal Eosinophils Interpretation



        Grade 1:
                 No cells seen
        Grade 2:
                 Only occasional eosinophils on smear.
        Grade 3:
                 Present but scanty and scattered throughout smear.
        Grade 4:
                 Approximately ¼ cells on smear eosinophils.
        Grade 5:
                 Approximately ½ cells on smear eosinophils.
        Grade 6:
                 Almost all cells on smear eosinophils.

Hope this helps.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073



----- Original Message -----
From: "Allen, Steven" 
To: 
Sent: Thursday, October 25, 2001 3:33 PM
Subject: Hansel's stain


> Hello,
>
> We are looking for a procedure for Hansel's stain.  Has anybody done this
in
> their lab, and do you have any hints/tips for this stain?Thanks in
advance!
>
> Steve Allen
> Lovelace Respiratory Research Institute
> 2425 Ridgecrest SE  Albuquerque, NM 87108
> (505)348-9159
>
>