Rachel/Linda
you are in effect forming a calcium hematoxylin, rather a calcium hematein that is insoluble.
Afraid I cannot offer a solution to this apart from decalcifying the section first or using a non hematoxylin
stain such as a trichrome.
Barry

LINDA MARGRAF MD wrote:

> Here's a message I am posting for   Rachel  while she works out the enclosure issue in her email system.
> She is on the list so you can reply to the list (just don't send the messages back only to me thanks, Linda M)
>
> 'Hi Everyone,
>
> I have been having a lot of trouble getting clean Haematoxylin and Eosin
> stains on my resin embedded undecalcified rat femur sections.  It seems as
> if the Heamatoxylin is forming some sort of precipitate on top of the
> calcified bone and it doesn't allow visualisation of the cellular structure.
>
> Does anyone have a working H&E stain for bone in resin?  Any ideas would be
> much appreciated.
>
> Thank you very much
>
> Rachel Peat
>
> Orthopaedic Research Dept
> Children's Hospital at Westmead
> Sydney, NSW, Australia'