This article has been very helpful for me:
"Adenoviral Expression of Vascular Endothelial Growth Factor -C Induces
Lymphangiogenesis in the Skin" Enholm et al 2001
(Circ Res. 2001; 88:623-629)

I am currently troubleshooting staining my FFPE mouse skin with RDI's
PECAM/CD31 rat anti-mouse monoclonal.
The paper above used tyramide amplification to enhance staining
(they used Pharmingens PECAM-1).
Also-When I contacted the researchers in Finland they said that they used
trypsin digestion (45min) - and no HIER.
So I'm testing with those perameters currently.

And to quote Gayle Callis from a previous post
"Murine CD markers often hate NBF much to mouse people's dismay and
frozens are the only recourse"
True, so true...
Our lab has great success with PECAM in Zinc (tris) fixed mouse skin.
But alas, I have an entire FFPE mouse experiment that I need to stain with
an endothelial cell marker.
I'm hoping for success with trypsin and TSA.


				Lara Muffley
                                Dermatology Dept
                                University of Washington
                                Seattle, WA
                                muffley@u.washington.edu

On Tue, 23 Oct 2001, Edwards, R.E. wrote:

>
>
> --
> I  am  looking  for  an  antibody  to  mouse  endothelial  cells  that
> works  on  paraffin  processed  tissues, any  suggestions  gratefully
> received.
>                                 Thanks
>                                        Richard  Edwards
>                                                    MRC TOXICOLOGY  UNIT
>                                                             LEICESTER
> U.K...........
>
>