EDTA decalcification with heat (can go up to 60C according to Brain's book,
but only if the bone is totally fixed).  This was known before microwave
methods came into being, and probably helps improve speed, be sure you
suspend the bone in the solution so decalcifier totally surrounds the jaw.
You should test the bone for endpoint, not so easy with EDTA, but possible. 



At 07:05 PM 10/23/01 +0000, you wrote:
>Any tips on using EDTA decal in the microwave to shorten the time?  The
>microwave allows for temperature control.  I seem to recall no higher than
>50 degrees, but cannot remember any time considerations.  Tissue is rat
>jaw.
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)