Hi Shannon
We are using Novocastra's clone 13H6 on FFPE, works like a charm - if you
are using HIER. HIER in 10 mM citrate buffer, pH 6.0 is okay, but we get
better results using a somewhat strange retrieval 'buffer', which is 100 mM
Tris - 5% Urea, pH 9.5; microwave. Working conc. for the antibody is 1 ug
Ig/ml (ask your supplier to provide the lot-specific Ig concentration when
you order; Novocastra has it and will provide the information upon request -
other companies do the same without the customer having to beg for it ...).
Good luck!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

Original message:
From: Klassen, Shannon SDH 
To: 'Histonet' 
Sent: Tue 23 Oct 2001 17:26
Dystrophins


> Hi all. Need some advice - I am currently using the dystrohin antibodies
> from Novocastra on frozen muscle sections (looking for Duchenne - Becker
> muscular dystrophy) and they work awesome but my pathologist would like to
> change antibodies so we can use the FFPE blocks. Now here is where my
> problem is - I always thought  that that using the frozen blocks for this
> was preferable as formalin fixation is not the greatest for preservation -
> True or not?
> You know the old adage - If it ain't broke - Don't fix it.  Anyone out
there
> using the FFPE antibody? Any help much appreciated. Thanks in advance.
> SHANNON KLASSEN
> IHC TECH
> SASKATOON DISTRICT HEALTH
> SASKATOON, CANADA
>