Hi Jenny,
I don't have information about that specific nuclei stain but I do use 
another fluorescence stain for nuclei: bis-Benzimide (Hoechst) . I mix 1 
mg of bis-benzimide in 1 mL distilled water. Then I take 4.0 micro 
liters of this stock and dilute with 1 mL PBS pH 7.4. The Sigma 
catalogue number for the bis-benzimide is B 2883.  I apply it to my 
cryostat sections for 10 minutes and rinse 3x for 5 minutes in PBS. The 
fluorescence color of the nuclei is bright blue and is viewed with a UV 
filter. The stock solution should be frozen (-20 C) and kept protected 
from light when not in use.
If you want a picture of what to look for let me know and I will email 
one directly to you.
Phillip Huff
FBN Dummerstorf
Germany

Jenny Molde wrote:

> Hi,
> We are looking for any information on how to make up the dapi counterstain
> for nuclei using Immunofluoresence? Any info will be greatly appreciatted.
> Many thanks.
> 
> Jenny Molde
> University of Cape Town
> Observatory
> Cape Heart Centre
> 
> ctsmolde@samiot.uct.ac.za
> 
> 
> 
>