Dear Margaret:
I suspect the recipé calls for Azure II-Eosinate, which is a sort of a salt of
the two dyes, Azure II and Eosin.  One is positively charged and the other
negatively charged.  If you mix aqueous solutions of the two, they sort of
combine, and the combination precipitates slowly out.  This is collected (by
paper filtration), washed with water, and dried.  It can then be dissolved in
an organic solvent (?methanol), and diluted to taste for the stain.  John
Kiernan's textbook explains it very nicely.
Good luck with the staining,
Adrian Leek.


SDSU HISTO wrote:

> Hi to all of you out in histoland.  I have been enjoying learning from this
> site.  I am making the Giemsa stain for Gaffney's Giemsa.  It calls for
> Azure II eosin in dry form.  Is this the same as Azure II from Sigma?
> Thanks
> Margaret Perry
> Lab Technician
> Box 2175
> Veterinary Science
> South Dakota State University
> Brookings, SD 57007
> sdsu_histos@sdstate.edu