Betsy, you might try spinning them down in warm agar, letting the button
solidify, tapping it out of the tube and embedding it "point" down.  You
might also want to tint the cells a little with hematoxylin or eosin so as
to better visualize them when sectioning.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu]
Sent: Wednesday, October 31, 2001 6:08 AM
To: histonet@pathology.swmed.edu
Subject: stem cells


Hi all,
I have received stem cells suspended in DMEM. They wanted to spin it down,
remove the pellet, process it for paraffin , embed and stain. Never having
dealt with suspension cells I could use any advice. 
Thanks to all.
Betsy Molinari
Texas Heart Institute
Houston,Texas 77030
713-794-6524