RE: microtome help

From:Connie McManus

At 02:44 PM 10/25/01 -0500, Sebree Linda A. wrote:
>I too have fond memories of using an old black AO; and no I don't think
>there is anything like it out there (or as good!). 

I remember these gems, too!  We have one sitting unused in our store room.
As far as I know, it still works.  

I use a Leica RM2145.  It has the ability to cut plastics as well as
paraffin, has 15 um and 30 um coarse trimming settings,  a 5 um setting and
a variable setting to cut any thickness you wish.  There is also a button
to cut in nm if you so desire, but so far I 've had no use for that.   I'm
very pleased with this microtome, but I don't think it is cheap.   You can
call Leica 800-248-0665 and get info.  Their website is www.leica.com  I
haven't found their website particularly easy to find what you're looking
for, but you can contact thm by e-mail from there.

happy hunting! *G*

Connie McManus


ur lab is currently
>using an Olympus Cut 4060 which does a pretty decent job on most specimens
>but I sometimes have problems with extremely dense tissue thick and thinning
>even after tightening everything, chilling the block and using a new blade.
>I think that one can buy a workhorse type microtome that can handle all
>tissues adequately but not necessarily elegantly or something like an
>Olympus that produces beautiful thin sections of things like lymph nodes but
>can't always stand up to all tissue types. 
>
>This is of course only my opinion after 20+ years in the field and I do not
>have experience with everything on the market these days. 
>
>As for price, I wouldn't expect to get a really good, new microtome cheaply.
>We paid $7,500 seven years ago for our instrument.
>
>Linda A. Sebree, HT(ASCP)
>University of Wisconsin Hospital & Clinics
>IHC/ISH Laboratory
>A4/204-2472
>600 Highland Ave.
>Madison, WI 53792-2472
>(608)265-6596
>FAX: (608)262-7174
>
>
>-----Original Message-----
>From: Julie Collins [mailto:jcollins@kumc.edu]
>Sent: Thursday, October 25, 2001 12:51 PM
>To: HistoNet@pathology.swmed.edu
>Subject: microtome help
>
>
>Can anyone tell me where I can get a decent inexpensive, new microtome?  I
>haven't purchased one in 10 years and have no idea where to start looking.
>I don't need anything fancy.  My favorite microtome I had was the old black
>AO.  Is there anything out there like that?
>Vendors are welcome to respond.
>
>TIA,
>Julie Collins
>Dir, Immunohistochemistry
>Univ. of Kansas Med. Center
>Kansas City, KS
>
>
>

Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA
(435) 797-1891
fax (435) 797-2805




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