RE: antigen retrieval

From:"Morken, Tim"

Veronique,

We use a steamer (20 minutes) to do our antigen retrieval, although we use
the microwave to heat the AR solutions. 

Tim Morken
CDC, Atlanta 


-----Original Message-----
From: =?UNKNOWN?Q?V=E9ronique?= Wunderle [mailto:vwunderle@yahoo.com]
Sent: Wednesday, October 24, 2001 3:37 AM
To: histonet@pathology.swmed.edu
Subject: antigen retrieval


hi everybody !
Being pretty new in the big world of
immunohistochemistry I am asking myself lots of
questions (some people tells me it's a good thing..). 
I know that I should get a good book on the subject
(any advice ?) but I find that "real-life" experience
is often much more informative.
So the question of the day is about antigen retrieval.
I have been looking around for different protocols and
most of them use a microwave oven. I can't buy right
now a microwave especially for IHC and I am a bit
concerned about setting up parameters on an oven used
extensively for other purposes. I am a bit anxious
that the life span of this oven is going to be pretty
short...
So, I tried the heating plate protocol (10mn in
boiling citrate buffer) on brain sections. So far, it
seems to be working pretty well. But I may be lucky.
I am therefore wondering if people out there tried
this and what you think about it. What make you prefer
the microwave method and here is the ultimate question
: is a microwave oven a "must have" for doing
incredible IHC ?
all your advices are welcome...
veronique.

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