RE: MyoD1

From:"Auld, John"


> Dear Linda
> 
> I use Nova Castra MyoD1 at 1/100 after pressure cooking in EDTA buffer pH
> 8.0 (0.1M). The control is Rhabdomyosarcoma. If its any consolation it
> didn't work the first couple of times, oh well the joys of immuno
> 
> Good luck
> 
> John
> 
> John Auld FIBMS MSc
> Biomedical Scientist 3
> Immunocytochemistry
> Department of Histopathology
> Royal Free Hospital
> Pond Street
> London NW3 2QG
> Tel 020 7794 0500 ext. 6516
> 
> 
> Date: 25 Oct 2001 13:01:54 -0500
> From: Lynde77627@aol.com
> Subject: MyoD1
> 
> I saw a note several weeks ago requesting help with MyoD1 but never saw
> any
> responses.  I am now in the process of attempting to get it to work.  If
> anyone has any insite to this antibody I sure would appreciate it.  I am
> using
> FFPE tissue and NovaCastra antibody.
> 
> Thanks in advance.
> Linda
> 
> 
> 




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