Hi all-

I am having a problem getting the basement membranes in the glomeruli to
stain.  The basement membranes of tubules and reticular fibers are staining
very well.  I'm using a kit from Polyscientific, Inc that recommends
incubation in at 70C for 60-75 minutes.  I've gone out to 90+ minutes with
no improvement.  I was hoping somebody out there would be able to shed some
light on the subject.  I'm going to another protocol that calls for
microwaving at 95C for 15 to 20 minutes but I wanted to see if there were
any other thoughts out there.

Thanks for the help.

Dave 
david.rosolia@aventis.com