NBT-BCIP

From:Luis Chiriboga

Hi all
Is anyone using the Ventana alkaline Phosphotase blue (NBT-BCIP) kit? If you
are,  how is working?  I have been having a lot of trouble getting good
signal/noise and avoiding the leaching artifact common with the method.  I
have even been getting precipitation of the NBT (even when using
stabilization buffer,  fresh ETOH, non-toluene based mounting media)  Any
help would be greatly appreciated.

Regards to all

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