From: | Luis Chiriboga |
Hi all Is anyone using the Ventana alkaline Phosphotase blue (NBT-BCIP) kit? If you are, how is working? I have been having a lot of trouble getting good signal/noise and avoiding the leaching artifact common with the method. I have even been getting precipitation of the NBT (even when using stabilization buffer, fresh ETOH, non-toluene based mounting media) Any help would be greatly appreciated. Regards to all --------------------------------------