Immunos on Bone Marrow

From:Slbearphix@aol.com

Does anyone have experience doing immunos on bone marrow, cytospins from
bone marrow or smears?
Due to the large number of red blood cells, it is difficult to see positively 
stained cells
using DAB. Is there  another way to lyse RBC besides methanol/peroxide?
I am trying to stain for macrophages in one satining procedure. 
I also need to stain erythrocytes. I need to do a double
staining method on the erythrocytes with co-localization within the same 
cells so I need to use 2 different colors.  Any suggestions?
Also, what is the best sources of anibodies for animal tissues?
Thanks in advance




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