From: | r-meyer2@northwestern.edu |
Does anyone know if 4% Paraformaldeyhde is the best fixative for cultured cells that will be paraffin embedded for in situ hybridization for mRNA? If so what is the time of fixation for these cells? I'm thinking probably 1 to 2 hours is plenty. If 4% Paraformaldehyde isn't the best fixative does anyone know which fixative will give the best results. Thanks Bob Meyer, HTL Northwestern University