ISH for mRNA

From:r-meyer2@northwestern.edu

Does anyone know if 4% Paraformaldeyhde is the best fixative for cultured cells 
that will be paraffin embedded for in situ hybridization for mRNA?  If so what 
is the time of fixation for these cells?  I'm thinking probably 1 to 2 hours is 
plenty.  If 4% Paraformaldehyde isn't the best fixative does anyone know which 
fixative will give the best results.  Thanks 

Bob Meyer, HTL 
Northwestern University 






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