Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: DDittus787@aol.com [mailto:DDittus787@aol.com]
Sent: Monday, October 29, 2001 4:19 PM
To: la.sebree@hosp.wisc.edu
Subject: Re: For Dana Dittus


Dear Linda

I believe we spoke about the presence of antigens on the surface of blood 
cells and creating agglutination in a test tube as in blood bank, there are 
many antibodies that hate heat, others that like cold, some we enzyme away, 
some we use enhancing agents to get them to show there presence in a
stronger 
way, I use this thinking in ihc somewhat , and in the way I approach certain

antibodies or groups of antibodies(ie:cytokeratins),While it is not exactly 
the same , since our tissue is preserved(formalin and alcohol,et al) it does

help me to usually achieve some success when doing new antibodies.I usuallt 
test my antibodies in a variety of ways, and then when similiar groups come 
along I start with what worked best in the past first. Hope this is helpful,

feel free to post on histonet if you like.
                                                         Dana