H&E for plastic

From:Linda Jenkins

Good Morning, Rachel!
	You are quite observant on the comment concerning the hematoxylin 
precipitate you observed on the surface of the plastic embedded slide -- 
this is precisely what happens with the routine paraffin "H&E" when applied 
to plastic sections.  Gayle and Evan are both correct when mentioning the 
methylene blue/basic fuchsin stain as an excellent substitute.  This stain 
requires etching. However, not all plastics etch the same.  Methyl 
methacrylate sections can be etched with a weak hydrochloric acid solution 
whereas a Technovit 7200 embedded slide will crack profusely.  These slides 
have to be etched with a weak formic acid solution.  So...several factors 
to consider; use the methylene blue/basic fuchsin instead of H&E (I will be 
glad to send you my version as well), which plastic used will influence the 
etching solution, and, as Gayle said, do not coverslip with mounting 
media.  I actually have found that adding a drop of immersion oil and then 
coverslippping before photography enhances the picture.  Remove coverslip 
afterwards and remove oil with a Kimwipe.
	Best wishes,
	Linda
P.S.  For Evan , et al.:  Cracking of certain plastic embedded sections is 
also a problem with light curing monomers such as Technovit 7200.  These 
must be stored in the dark as room light will continue to slowly polymerize 
the section causing cracking and bowing of the plastic section. 





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