Hello to Everyone,

I am not new to frozen sectioning, but I am new to cutting frozen bone 
sections.  Any help to prevent me from pulling out more hair would be 
greatly appreciated.

The first question is, are decent frozen bone sections possible without 
using the tape transfer system?  I have seen that there has been some 
debate about this on histonet in the past.  The tissue I am trying to cut 
is rabbit tibia as practice for a small upcoming human project.  I have 
tried a range of temperatures, but -30 to -35 degrees Celsius seems to be 
the suggested temperature based on the reading I have done.  I am also 
using a tungsten carbide knife and Superfrost Plus slides.  I was hoping 
for sections in the 7-10 um range.  The problems are that I am getting 
shattered sections and even when I try to pick up a section, the actual 
bone does not want to stick to the slide.  I have not yet tried other 
coated slides, mind you this would only eliminate the adherence 
problem.  Any suggestions?!

As always, I am exceptionally grateful for any help you may provide.

Tracey Couse
Petit Institute for Bioengineering & Bioscience
Georgia Institute of Technology