Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: DDittus787@aol.com [mailto:DDittus787@aol.com]
Sent: Saturday, October 27, 2001 8:43 AM
To: la.sebree@hosp.wisc.edu
Subject: Re: For Dana Dittus


Dear linda
No problem, I assume you want me to explain , how we fix our cytospins for 
retrieval purposes?  We have fixed in formalin, acetone/alcohol  50/50  or 
alcohol only.This depends on the antibody that needs testing. I usaually fix

at room temp for 10-15 minutes then allow to air dry and proceed with any
set 
retrieval protocol that the antibody requires. I have found all methods 
eliminate extraneous blood(water contents rupture cells) formalin and 
alcohols fix white and malignant cells to slide.For example since 
cytokeratins are a popular request after fixation we do protease on our 
instrument for 8 min and then stain, if ER/PR is requested , then formalin 
fix and then microwave retrieve, etc. If I can be of any additional help , 
you can e-mail me at work,(just got it DDittus@amh.org)  or contact me by 
phone 215-481-2609. Feel free to post this on histonet if you like.

       Dana