For the person anticipating antigen retrieval, I advise buying this book  

Antigen Retrieval Techniques: Immunohistochemistry and molecular morphology

SR Shi, J Gu and Clive Taylor

Eaton Publishing, can buy it from BioTEchniques Books Publications, try
their website for direct purchase www.BioTechniques.com

ISBN #1-881299-43-0

I don't do retrieval in research protocols, but own the book, it is a 350
page Pearls of Wisdom text on all aspects of retrieval by the experts.  It
will explain basics and give protocols.  

 

 
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)