Dear netters,

I thank all of you who responded to my staining problem.  I realize this
technique is a hot topic and its outcome is often in question, but we are
here to please our researchers!  Also, I'd like to give a special thanks to
Frances Swain of the University of Arkansas for taking time out of her day
to discuss this problem with me at length.

thanks again,

Tom Galati
Histology Laboratory Director
HSRL-A GLP Compliant Laboratory
137 S. Main Street
Woodstock, VA  22664
(540)459-8211
fax: (540)459-8217
tomgalati@hsrl.org
www.hsrl.org
----- Original Message -----
From: "rueggp" 
To: "Histo-Scientific Research Laboratories" 
Sent: Saturday, September 29, 2001 1:38 AM
Subject: Re: TUNEL Expertise Needed


> Tom,
> make sure the problem is fixation and processing, i often get nonspecific
> staining even with well tended tissue, most people i talk to have trouble
> distinquishing apoptosis from necrosis with the tunnel method.
> patsy ruegg
>
> Histo-Scientific Research Laboratories wrote:
>
> > Dear Histonetters,
> >
> > We have a researcher that had sheep myocardial tissue that upon necropsy
he
> > put in 70%ETOH.  When he went to do his TUNEL staining, he noted
nonspecific
> > staining rendering his project useless.  Would he be able to 1) cut
sections
> > from the processed tissue and post-fix the slides in formalin or
> > paraformaldehyde to make his stain work?  2) If not, could he take the
> > remaining wet tissue that is currently in 70%ETOH and put it in a
fixative
> > then process as normal and get results with his TUNEL staining?   Your
help
> > is greatly appreciated.
> >
> > Sincerely,
> >
> > Tom Galati
> > Histology Laboratory Director
> > HSRL- A GLP Compliant Contract Laboratory
> > 137 S. Main Street
> > Woodstock, VA  22664
> > (540)459-8211
> > fax: (540)459-8217
> > tomgalati@hsrl.org
> > www.hsrl.org
>